Part:BBa_K133100:Design

CMV-SS-HA-CD4sh-(TIR)TLR3-(TIR)TLR9-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1307
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2406
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1920
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1754
  Illegal BsaI site found at 2756
  Illegal SapI.rc site found at 842
  

Design Notes

The part is cloned into BBa_J52017, a vector with eukaryotic terminator.